Figure 4.

Effect of BLT1 receptor antagonist CP-105,696 on LTB₄-mediated retardation of human neutrophil apoptosis. Neutrophils (5 × 10⁶ cells ml⁻¹) were cultured in serum-supplemented Iscove's MDM alone (control, a) or in the presence of LTB₄ (1 μM, b). Neutrophils were resuspended following 20 h in culture and incubated with FITC-labelled recombinant human Annexin V to determine phosphatidylserine expression. The cells were then fixed and analysed using an EPICS Profile II. Mean fluorescence values are shown for a minimum of 5000 cells for each condition. (c) Neutrophils (5 × 10⁶ cells ml⁻¹) were preincubated (37°C, 15 min) in serum-supplemented Iscove's MDM containing BSA (0.1%) and treated with LTB₄ (1 μM) with or without CP-105,696 (3 μM). Samples were harvested to assess for morphological features of apoptosis; data represent the means±s.e.m. of three independent experiments performed in triplicate, where ^* represents P<0.05 compared to control values.