Figure 7.

Effect of the Cys-LT₁ receptor antagonist montelukast on agonist-induced calcium fluxes in human eosinophils. Cytosolic calcium measurements (nM) in fura-2-loaded eosinophils (2 × 10⁶ cells ml⁻¹ in HBSS without phenol red, with CaCl₂ and MgCl₂) over 540 s. Buffer alone (control, a) or montelukast (1 μM, b) was added to the cell suspension after 60 s, followed by the sequential addition of LTD₄ (100 nM), LTB₄ (100 nM) and PAF (100 nM) after 245, 365 and 485 s, respectively. The data show traces obtained from a typical experiment performed at least three times.