Figure 2.

Inhibitory effects of gliclazide on (a) 10 μM pinacidil- and (b) 500 μM diazoxide- induced inward membrane currents at −50 mV in symmetrical 140 mM K⁺ conditions by use of nystatin-perforated patch configuration. The dashed line indicates zero current. (c) Relation between relative inhibition of the peak amplitude of each K_ATP channel opener (pinacidil (10 and 100 μM) and 500 μM diazoxide)-induced current and the concentration of gliclazide. The following values were used for the curve fitting: 10 μM pinacidil, K_i=177 μM, h=0.8; 100 μM pinacidil, K_i=285 μM, h=0.8; diazoxide, K_i1=5 nM, h1=1.2; K_i2=108 μM, h2=0.7, L=0.74. Each symbol indicates the mean of 3–8 observations with s.d. shown by vertical lines.