Figure 1.
Agarose gel showing products of reverse-transcriptase–polymerase chain reaction (RT–PCR) assay for cDNA from uterine samples from (a) a nonpregnant premenopausal woman and (b) a pregnant woman. Single transcripts corresponding to the sizes predicted for the NK1 receptor (NK1R), the NK2 receptor (NK2R), PPT-B and NEP were detected in all the tissues. The specific bands corresponding to the tachykinin NK3 receptor (NK3R) and PPT-A were only detectable in nonpregnant human uteri. The mRNA of PPT-C was not detected after amplification of the usual amount of uterine cDNA. β-actin was used as housekeeping gene. M, molecular size standards. Data are typical of results in five pregnant and five nonpregnant women.