Table 1.
Sequences of forward (F) and reverse (R) primers of indicated target genes and the size expected for each PCR-amplified product. Primers for β-actin, used as an internal control, are also shown
| Gene | Forward primer | Reverse primer | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| PPT-A | 5′-TGTGTCAGCTACTGCGGAAA-3′ | 5′-CAATGAAATGAAGACATTAGAATCACA-3′ | 193 | Harmar et al. (1986) |
| PPT-B | 5′-CCCCCGAGAGCAGAATAGGT-3′ | 5′-CCAGGGTCAGGTAGAAAAAGATGG-3′ | 171 | Page et al. (2000) |
| PPT-C | 5′-CCTYGCCCTGYTTCTCCT-3′ | 5′-YCCACYCGCTTCCCCATC-3′ | 204 | Zhang et al. (2000) |
| NK1R | 5′-GCTGCCCTTCCACATCTTCTT-3′ | 5′-GTCCTCTGGCTCCTCCTCGT-3′ | 331 | Takeda et al. (1991) |
| NK2R | 5′-GCCCTACCACCTCTACTTCATCC-3′ | 5′-AGCAAACCATACCCAAACCA-3′ | 375 | Gerard et al. (1990) |
| NK3R | 5′-TTGCGGTGGACAGGTATATGG-3′ | 5′-GGCCATTGCACAAAGCAGAG -3′ | 178 | Takahashi et al. (1992) |
| NEP | 5′ -AGCCTCTCGGTCCTTGTCCT-3′ | 5′-GGAGCTGGTCTCGGGAATG-3′ | 219 | Shipp et al. (1988) |
| β-actin | 5′-TCCCTGGAGAAGAGCTACGA-3′ | 5′-ATCTGCTGGAAGGTGGACAG-3′ | 362 | Nakajima-Iijima et al. (1985) |
NEP, neprilysin.