Table 1.
Peptide sequences against which the antibodies used in these experiments were raised
| Internal sequence | C-terminal sequence | |
|---|---|---|
| Gαi1 | LDRIAQPNYI (159–168) | |
| Gαi1/2 (Calbiochem) | KNNLKDCGLF | |
| Gαi1/2 (NEN*) | KENLKDCGLF | |
| Gαi3 | KNNLKECGLY | |
| Gαi3/o | KNNLKECGLY (Calbiochem) | |
| ANNLRGCGLY (NEN) | ||
| GαoGαq/11 | Full-length recombinant Gαo | QLNLKEYNLV |
| Gαs | RMHLRQYELL | |
| Gβ | KTREGNVRVSREL (127–139) |
The single letter code for amino acids is used. The same decapeptide (KNNLKECGLY) was used by Calbiochem to raise antibodies against Gαi3 or Gαi3/Gαo. Antibodies selective for either the Gαi3 or selective for both Gαi3 and Gαo were obtained. NEN, however, used a mixture of the C-terminal end of Gαo (ANNLRGCGLY) and the C-terminal end of Gαi3 (KNNLKECGLY) to produce an antibody which recognized both of these α subunits. The polyclonal anti-Gβ antibody recognizes both 35 and 36 kDa subunits.
*
Gαi1/Gαi2(NEN) was raised using the C-terminal sequence of the transducin α subunit (KENLKDCGLF).