Table 2.
Imax (nA) | EC50 (μM) | h | |
---|---|---|---|
Antibodies experiments | |||
Control | 0.857 | 8.1 | 1.0 |
Anti-Gαi3/Gαo (Calbiochem) | 0.338 | 4.3 | 1.18 |
Anti-Gαi3/Gαo (NEN) | 0.165 | 8.1 | 0.79 |
Anti-Gαo (Calbiochem) | 0.229 | 8.0 | 1.65 |
Anti-Gαi1/Gαi2 (Calbiochem) | 0.783 | 4.4 | 2.15 |
Anti-Gαi1/Gαi2 (NEN) | 0.891 | 6.2 | 0.54 |
Anti-Gαi1 (Calbiochem) | 0.994 | 10.8 | 1.29 |
Anti-Gαi3 (Calbiochem) | 0.914 | 8.0 | 1.30 |
Anti-Gαs (Calbiochem) | 1.050 | 3.1 | 1.57 |
Anti-Gαq/Gα11 (NEN) | 0.862 | 5.7 | 0.58 |
Gβγ experiments | |||
Control | 0.921 | 2.4 | 1.3 |
Gβγ | 0.804 | 2.4 | 1.1 |