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. 2003 Jun 9;139(3):605–615. doi: 10.1038/sj.bjp.0705289

Table 2.

The maximum amplitude (Imax), 50% effective concentration (EC50) and Hill slope (h) of the carbachol concentration–response curves for mIcat activation in control and anti-G protein antibody-treated cells and in Gβγ-treated cells

  Imax (nA) EC50 (μM) h
Antibodies experiments      
  Control 0.857 8.1 1.0
  Anti-Gαi3/Gαo (Calbiochem) 0.338 4.3 1.18
  Anti-Gαi3/Gαo (NEN) 0.165 8.1 0.79
  Anti-Gαo (Calbiochem) 0.229 8.0 1.65
  Anti-Gαi1/Gαi2 (Calbiochem) 0.783 4.4 2.15
  Anti-Gαi1/Gαi2 (NEN) 0.891 6.2 0.54
  Anti-Gαi1 (Calbiochem) 0.994 10.8 1.29
  Anti-Gαi3 (Calbiochem) 0.914 8.0 1.30
  Anti-Gαs (Calbiochem) 1.050 3.1 1.57
  Anti-Gαq/Gα11 (NEN) 0.862 5.7 0.58
Gβγ experiments      
  Control 0.921 2.4 1.3
  Gβγ 0.804 2.4 1.1

These parameters were determined by curve fitting of individual sets of data points in Figures 3 and 6d so that the values slightly differ from those in the text, which are the averaged values of the maximum responses (see Methods).