Figure 1.

Bethanechol- and CCK-stimulated amylase secretion was blocked by atropine and FK480, respectively. Freshly isolated pancreatic acini were perfused. In (a), bethanechol 100 μM (circles, n=4) or bethanechol 100 μM plus atropine 10 μM (diamonds, n=3) was added as indicated by the horizontal bar. In (b), CCK 100 pM (circles, n=4) or CCK plus FK480 1 μM (diamonds, n=4) was added as indicated by the horizontal bar. Data are presented as m±s.e.m. Note the complete blockade of amylase secretion with addition of atropine in (a) and of FK480 in (b). Some standard error bars lie within the symbols.