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. 2003 Jun 20;139(4):765–774. doi: 10.1038/sj.bjp.0705303

Figure 1.

Figure 1

Effect of retinoic acid on the mRNA expression of ET-1 and its receptors in HSC. Cells were incubated with retinoic acid (2.5 μM) or the vehicle (ethanol, 1 μl ml−1) for 10–12 days, after which RNA was extracted and the mRNA expression of preproET-1, ETA and ETB was determined by RT–PCR. (a) mRNA expression of various transcripts from a representative experiment. (b) mRNA expression of the respective transcripts normalized with respect to the expression of β-actin mRNA. Values are means of six separate determinations±s.e.m. *P<0.05 vs vehicle.