Figure 6.

Effect of retinoic acid and ET-1 or sarafotoxin S6c on protein synthesis. (a) HSC were incubated with 2.5 μM retinoic acid or the vehicle (ethanol, 1 μl ml⁻¹) in the absence or presence of ET-1 (50 nM) or sarafotoxin S6c (SFX) (50 nM) for 10–12 days. In additional wells, cell were incubated with ET-1 and 10 μM BQ-123 or BQ-788. [³H]leucine incorporation and DNA measurements were made as described in the Methods section. ^*P<0.01 vs vehicle; ^**P<0.001 vs control. (b) HSC were incubated with retinoic acid or vehicle alone for 10–12 days. ET-1 or sarafotoxin S6c were then added and 24 h later DNA synthesis ([³H]leucine incorporation) and DNA concentration (Hoechst's reaction) were measured. ^*P<0.01 vs vehicle; ^**P<0.001 vs control. SFX, sarafotoxin S6c.