Table 1.
Measurements of product abundance
| DST |
MspI
|
Sau3AI
|
Northern
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| −S | +S | +/− | −S | +S | +/− | −S | +S | +/− | |
| NF-κB | 111 | 764 | 6.9 | 211 | 1,330 | 6.3 | 0.70 | 6.5 | 9.3 |
| OS51 | 159 | 1,880 | 11.8 | 35 | 1,820 | 52 | 0.17 | 3.74 | 22.0 |
The peak amplitudes of the two DSTs under study from the serum-depleted (−S) and serum-replenished (+S) samples were determined from the MspI and Sau3AI browser panels, and their ratios (+/−) were calculated. The Northern blots contained, in addition to the 2-μg poly(A)-enriched RNA samples from the serum-depleted or serum-replenished cells, a dilution series of cRNAs produced from the isolated, cloned DSTs. The intensities of the hybridization signals were compared to determine the mass ratio, expressed as parts per 100,000.