Table 1.
Effects of various cannabinoid receptor ligands on IL-1β and TNF-α secretion by stimulated THP-1 cells
| Ligand | Concentration (μM) | IL-1β secretion (% control) | P | TNF-α secretion (% control) | P |
|---|---|---|---|---|---|
| Δ9-THC | 10 | 55±13 (5) | <0.01 | 77±2 (3) | 0.07 |
| 5 | 64±7 (5) | 90±3 (3) | |||
| 1 | 92±5 (5) | 105±15 (3) | |||
| Δ8-THC | 10 | 53±11 (5) | 0.03 | 79±26 (3) | 0.07 |
| 5 | 69±7 (5) | 89±20 (3) | |||
| 1 | 92±5 (5) | 107±22 (3) | |||
| Anandamide | 10 | 64±11 (5) | <0.01 | 87±20 (3) | 0.2 |
| 5 | 87±4 (5) | 112±5 (3) | |||
| 1 | 90±7 (5) | 106±12 (3) | |||
| BML-190 | 10 | 101±7 (5) | 0.48 | 147±28 (4) | 0.04 |
| 5 | 101±5 (5) | 146±20 (4) | |||
| 1 | 100±3 (5) | 140±13 (4) | |||
| JWH-015 | 10 | 69±11 (4) | <0.01 | 84±8 (4) | 0.03 |
| 5 | 81±11 (4) | 81±9 (4) | |||
| 1 | 89±8 (4) | 105±6 (4) |
Drugs were administered to THP-1 cells 30 min before stimulation with a mixture of LPS (0.5 μg ml−1) and IFN-γ (150 U ml−1). IL-1β and TNF-α concentrations in cell-free supernatants were measured 48 h later. Data (means±s.e.m.) are expressed as percent change in cytokine concentrations, with mean absolute values obtained from stimulated cells in the presence of corresponding vehicle solutions being 1.3±0.5 U ml−1 for IL-1β and 6.8±2.6 ng ml−1 for TNF-α. The number of independent experiments for each set of data is shown in parentheses. The concentration-dependent effects of various drugs were assessed by randomized blocks design ANOVA and P-values obtained for each of the drug treatments are presented. Significant P-values are indicated in bold type.