Figure 5.

Effect of ET-1 on TNFα-induced iNOS mRNA expression in differentiated 3T3-F442A adipocytes. Differentiated 3T3-F442A cells were treated for 20 h with or without TNFα in the presence or absence of ET-1 (10 nM). (a) mRNA extracts were analysed by RT–PCR. A representative autoradiography taken from two independent experiments is shown. (b) mRNA were also analysed by Northern blot. A representative autoradiography is shown. (c) Results of densitometric analysis are presented and expressed as mean±s.e.m. from two independent experiments (the radioactivity was normalised with the corresponding signal obtained with β-actin).