Table 1.
Effects of various inhibitors on the inhibition produced by ET-1-on TNFα-induced nitrite accumulation in differentiated 3T3-F442A
| Inhibition of TNFα-induced nitrite accumulation (%) | |
|---|---|
| ET-1 (10 nM) | 74±17 |
| ET-1+indomethacin (10 μM) | 84±5 |
| ET-1 (10 nM) | 78±8 |
| ET-1+bisindolylmaleimide (10 μM) | 72±9 |
| ET-1 (10 nM) | 64±1 |
| ET-1+SB 203580 (10 μM) | 67±5 |
| ET-1+wortmannin (50 μM) | 39±6* |
| ET-1 (10 nM) | 64±7 |
| ET-1+LY 294002 (1 μM) | 63±11 |
| ET-1+LY 294002 (3 μM) | 74±9 |
| ET-1+LY 294002 (10 μM) | 84±6* |
Data are shown as mean±s.e.m. of three to four experiments performed with cells studied at three different passages (in duplicate for indomethacin and bisindolylmaleimide or quadruplicate for SB 203580, wortmannin and LY 294002). *indicates a statistically significant difference (ANOVA followed by a Dunnett's test for multiple comparison test, P<0.05).