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. 2003 Sep 3;140(1):41–52. doi: 10.1038/sj.bjp.0705398

Figure 4.

Figure 4

Effect of TNF-α on apoptotic index of SCBN cells in the presence or absence of PKC isoenzyme activators and inhibitors. Apoptotic index was assessed as the percentage of cells displaying nuclear condensation and fragmentation. A total of at least 300 cells were counted in each experiment. (a) Changes of apoptotic index in SCBN cells treated with TNF-α and the effect of a 30-min pretreatment with PKC isoform peptide inhibitors, pp95 (0.5 μM), cPKC antagonist; pp101 (0.5 μM), PKCδ antagonist; pp93 (0.5 μM), PKCɛ antagonist. Cells were examined 18 h after TNF-α treatment. (b) Comparison of the effect of PKC agonists, pp111 (0.5 μM), a cPKC agonist; pp114 (0.75 μM), a PKCδ agonist and pp106 (0.75 μM), a PKCɛ agonist and antagonist peptides with that of TNF-α challenge alone on apoptotic index. Data are means±s.e. of three separate experiments each performed in duplicate. *P<0.05, significant difference compared to control groups; +P<0.05, significant difference compared with TNF+AMD groups; ++P<0.05, significant difference compared with corresponding agonist peptides group, respectively, as determined by ANOVA and Dunnett's test.