Figure 7.

Effect of PKC selective isoenzyme peptide activators and inhibitors on I-κBα protein levels in SCBN cells treated with TNF-α (10 ng ml⁻¹) in the presence of AMD (2 μg ml⁻¹). SCBN cells were pretreated for 1 h in the presence or absence of PKC inhibitors, before specific agonists are applied. Quantitative analysis of IκBα protein changes was derived from densitometric analysis of autoradiograms from the treated cells. Values are mean±s.e. of data from three separate experiments. ^*P<0.05 compared with control; ⁺P<0.05 compared with agonist peptide-treated group. Reprobe of the same blot with actin monoclonal antibody showed equal loading.