Table 5.

PTx sensitivity of agonist-stimulated GTPγ³⁵S binding and cAMP inhibition for CHO_INDhNOP membranes and cells (5 μM ponasterone A), respectively

Ligand	GTPγ35S binding (stimulation factor)		cAMP inhibition (%)
	Control	+PTx	Control	+PTx
N/OFQ	2.45±0.34	1.01±0.16*	43.1±8.9	0.0±9.6*
N/OFQ(1–13)–NH2	2.49±0.38	1.17±0.13*	50.3±8.8	1.7±7.0*
[F/G]N/OFQ(1–13)–NH2	1.48±0.13	0.74±0.20*	45.5±16.7	3.4±15.3*

Agonists were included at 10 μM for GTPγ³⁵S experiments and 100 nM for cAMP measurements. Data are mean±s.e.m.; n⩾3.

^*Values are significantly reduced compared with control, P<0.05 (unpaired t-test).