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. 2000 Feb 11;97(5):1982–1987. doi: 10.1073/pnas.030539397

Figure 2.

Figure 2

Expression of Nkx-1.2 mRNA. (Upper Left) Northern analysis. mRNAs from adult mouse tissues blotted on Nylon membranes (CLONTECH) were hybridized following the manufacturer's instructions, with a 300-bp 32P-labeled DNA probe containing the Nkx-1.2 coding region after the homebox (residues 7823–8123 in Fig. 1). (Upper Right) RNase protection assay. Poly(A)+ RNA preparations were hybridized with a 32P-labeled Nkx-1.2 cRNA probe (nucleotides 7623–8123 in Fig. 1) and processed according to the RPA-II system instructions (Ambion). The autoradiogram was exposed overnight. (A–D) In situ hybridization. Serial sections from mouse embryos 14 days after fertilization were hybridized with a 500-nt residue (7623–8123 in Fig. 1), 35S-labeled Nkx-1.2 antisense (A–C) or sense (D) RNA probe, and slides were contact-exposed for 15 days. mo, medulla oblongata; p, pons; p/m, pons/mesencephalon boundary; dt, dorsal thalamus; sc, spinal cord; g, gut; ge, genital eminence; v, vertebrae.