Figure 6.

Nkx-1.2 gene expression in adult brain. (A) Whole brain, coronal. (B) Pons, diencephalon. (C) Cerebral cortex. (E) Piriform cortex. (F) Cerebellum; cerebellar cortex. In situ hybridization was performed as described in Materials and Methods using Nkx-1.2 RNA probes labeled with [³³P] corresponding to an EcoRI–BamHI nucleotide sequence, approximately 850 bp in length, starting at the EcoRI site located at residues 8119–8123 in Fig. 1. Shown are dark-field images of sections hybridized with the antisense NKx-1.2 cRNA probe; little or no hybridization signal was obtained with the sense probe (data not shown). RspC, retrosplenial cortex; PPAC, posterior parietal associative cortex; SSC, somatosensory cortex; Pir, piriform cortex, Amgd, amygdaloid body; CA, cornu ammoni; DG, dentate gyrus; Zi, zona incerta; mHab and lHAb, medial and lateral habenula, respectively; DMH and VHM, dorsomedial and, ventromedial hypothalamic nucleus, respectively; Arc, arcuate nucleus; X, dorsal motor nucleus vagus; VIIIcoch, ventral and dorsal cochlear nuclei; VIII vest, vestibular nucleus; VII, facial nucleus; V, trigeminal nucleus; RP, raphe pallidus.