Table 3.
Amino acid | Control | Control(−L-arginine) | LPS+IFN-γ | LPS+IFN-γ(−L-arginine) |
---|---|---|---|---|
L-arginine (mM) | 1.43±0.36 | 0.26±0.01* | 0.76±0.10 | 0.30±0.03⧫ |
L-citrulline (mM) | 0.16±0.03 | 0.49±0.29 | 3.19±0.11* | 0.04±0.04⧫ |
Unstimulated and activated aortic smooth muscle cells (100 μg ml−1 LPS+50 U ml−1 IFN-γ, 24 h) were extracted and intracellular L-arginine and L-citrulline concentrations determined by reverse-phase hplc. Results are expressed as intracellular amino-acid concentration (mM) assuming an intracellular water space of 1 pl. Data denote the means±s.e.m. of determinations in three different cell cultures.
P<0.05 versus control
P<0.05 versus LPS+IFN-γ.