Figure 7.

Block of Thg-induced inward current. (a) Trivalent cations La³⁺ and Gd³⁺ block Thg-induced inward current; this block could be reversed by chelating lanthanides with EGTA. (b) 2-APB caused partial and reversible block of the Thg-induced inward current, while replacement of all extracellular permeable cations by NMDG⁺ abolished the current completely. (c) Single channels underlying Thg-induced whole-cell current were revealed in the presence of 2-APB, which reduced the opening frequency without affecting conductance. No activity could be observed in NMDG⁺-based solution. Holding potential −80 mV, intracellular solution – Cs methanesulphonate, 10 mM BAPTA. Lines indicate zero current level. Traces shown are typical of six to experiments.