Table 1.
Oligonucleotide sequences used for real-time PCR amplification
| Gene | Oligonucleotide | Sequence | PCR product size (bp) |
|---|---|---|---|
| 18S RNA | Forward primer | ACGGCTACCACATCCAAGGA | |
| Reverse primer | AAGGATTTAAAGTGGACTCATTCCA | 100 | |
| Probe | CGCGCAAATTACCCACTCCCGA | ||
| ETA-R | Forward primer | CAGCCACAAGGACAGCATGA | |
| Reverse primer | TGCTGCGTGACCGTTTCA | 86 | |
| Probe | CCGAGCAGTGTGCCTTCGAGTCC | ||
| ETB-R | Forward primer | CCTGCGGAGGTGACCAAA | |
| Reverse primer | GGCATGATACAATCGTGTTGATG | 124 | |
| Probe | AGTCCCGCCAAGATCCTTCCCTCC | ||
| ECE | Forward primer | GAGAAGCGCCGGGATGA | |
| Reverse primer | GGCATTCAGAAAGGGTAACCAG | 99 | |
| Probe | AGCTCATCTACCACAAAGTCACGGCTGC | ||
| ppET-1 | Forward primer | TTGATGGACAAGGAGTGTGTCTACTT | |
| Reverse primer | TTCCTAGTCCATACGGGACGAC | 88 | |
| Probe | CCACCTGGACATCATCTGGGTCAACA |