Table 3.
The relative expression of target genes in neonatal cardiomyocytes stimulated for 24 h with E2 (10−8 M) or vehicle (control) obtained by real-time PCR determined as ΔCT
| ETA-R | ETB-R | ppET-1 | ECE | |
|---|---|---|---|---|
| Control | 9.25±0.23 | 14.11±0.23 | 10.76±0.24 | 7.58±0.25 |
| E2-stimulated | 9.41±0.23 | 14.84±0.27 | 10.51±0.54 | 7.66±0.27 |
ΔCT were first obtained as ratios between the target gene CT and the reference gene 18S rRNA CT for each experiment to correct for variations in amount of cDNA. Thereafter, ΔCT were averaged for each treatment group (mean±s.d.).