Figure 2.

Expression of WT and mutant 5-HT_3A receptors. (a) Normalized whole-cell recordings of the responses to 10 μM 5-HT (solid bar, 30 s) in HEK293 cells expressing WT, EA, SR or EASR mutant receptors. (b) Typical immunofluorescence labelling of nonpermeabilised HEK293 cells expressing the AK mutant receptor with an antiserum specific for the N-terminal region of 5-HT₃ receptors. Scale bar, 10 μM. (c) Typical maximal increases in the intracellular Ca²⁺ concentration induced by 10 μM 5-HT (solid bar) in individual HEK293 cells loaded with Fura-2 expressing WT or mutant receptors.