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. 2003 Oct 1;140(3):547–557. doi: 10.1038/sj.bjp.0705464

Figure 7.

Figure 7

JNK activity is required for THC-induced DNA fragmentation. (a) Exposure of neurons to THC (5 μM) for 3 h significantly increased the percentage of cells with DNA fragmentation. Treatment with JNK1 AS or JNK2 AS (2 μM; 48 h) prior to THC treatment prevented the THC-induced increase in DNA fragmentation. In contrast, in neurons treated with JNK1 SC or JNK2 SC (2 μM; 48 h), THC still evoked a significant increase in DNA fragmentation. Results are expressed as mean±s.e.m. for five observations, ***P<0.001. (b) Representative images of (i) control, (ii) THC-treated and (iii) THC/JNK1 AS-treated cortical neurons following TUNEL staining for DNA fragmentation. Arrows indicate TUNEL-negative (−ve) and TUNEL-positive (+ve) cells. Scale bar is 50 μM. (c) Representative images of TUNEL staining of (i) control, (ii) THC-treated and (iii) THC/JNK2 AS-treated cortical neurons. Scale bar is 50 μM.