Table 1.
Heart rate and coronary flow in the isolated perfused rat heart
| Baseline | Ischaemia | Reperfusion | |||||
|---|---|---|---|---|---|---|---|
| Treatment | N | HR | CF | HR | CF | HR | CF |
| CON | 8 | 241±06 | 13.1±0.9 | 217±10 | 5.9±0.6 | 211±11 | 8.1±1.1 |
| UP | 9 | 232±10 | 11.6±1.6 | 215±08 | 5.2±1.7 | 209±07 | 7.8±0.9 |
| BNI+UP | 6 | 237±13 | 12.6±1.4 | 212±11 | 6.7±1.1 | 207±10 | 10.2±2.1 |
| BNI | 6 | 229±09 | 11.8±1.1 | 208±07 | 4.8±0.8 | 210±08 | 9.8±0.7 |
| HMR+UP | 9 | 239±07 | 13.2±0.8 | 211±09 | 7.2±1.5 | 209±12 | 11.2±0.6 |
| 5-HD+UP | 8 | 247±12 | 14.3±1.0 | 220±10 | 7.8±1.2 | 212±07 | 12.8±1.1 |
| UP+HMR | 9 | 251±08 | 14.1±0.6 | 228±08 | 6.9±0.7 | 200±05 | 13.2±0.5 |
| UP+5-HD | 6 | 226±16 | 10.7±1.2 | 210±12 | 4.9±0.9 | 211±09 | 8.7±1.6 |
| UP+Glib | 8 | 235±06 | 12.5±0.6 | 218±10 | 7.1±1.0 | 222±09 | 8.3±0.8 |
| 5-HD(before) | 6 | 242±09 | 11.6±0.8 | 230±14 | 4.9±0.6 | 232±05 | 10.1±1.6 |
| HMR (end) | 6 | 238±11 | 12.1±0.9 | 224±13 | 6.4±1.3 | 221±06 | 9.7±0.9 |
| 5-HD(end) | 6 | 245±10 | 14.5±1.0 | 219±10 | 7.0±0.4 | 213±12 | 12.4±1.8 |
| Glib(end) | 6 | 233±09 | 13.2±1.1 | 212±10 | 7.7±1.2 | 223±11 | 9.8±1.2 |
The experimental protocol was described in Figures 1, 2,3,4,5. Baseline, at 10 min of perfusion; Ischaemia, 5 min after regional ischaemia; Reperfusion, 120 min after reperfusion; CON: administration of saline 24 h before I/R; UP: administration of U50,488 H (10 mg kg−1) 24 h before I/R; BNI+UP: administration of nor-BNI (10 mg kg−1) 10 min before U50,488 H (10 mg kg−1) administration; BNI: nor-BNI (10 mg kg−1) was given intravenously 10 min before administration of saline 24 h before I/R; HMR+UP or 5-HD+UP: administration of U50,488 H (10 mg kg−1) together with HMR-1098 (6 mg kg−1) or 5-HD (10 mg kg−1) 24 h before I/R; UP+HMR, UP+5-HD or UP+Glib: perfusion with HMR-1098 (30 μM), 5-HD (100 μM) or Glib (10 μM) alone 20 min before I/R at 24 h after administration of U50,488 H (10 mg kg−1); HMR (before) or 5-HD (before): administration of HMR-1098 (6 mg kg−1) or 5-HD (10 mg kg−1) with saline 24 h before I/R; HMR (end), 5-HD (end) or Glib (end): perfusion with HMR-1098 (30 μM), 5-HD (100 μM) or Glib (10 μM) alone for 20 min before I/R at 24 h after intravenous administration of saline. HR, heart rate in beats per min; CF, coronary flow in ml min−1. Values are means±s.e.m.; n is the number of hearts.