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Indirect immunofluorescence staining of p65/NF-κB in HASMC cultures treated for 1 h with basal vehicle medium, 10 nM of either N-Hb or E-Hb, or 10 ng ml−1 TNF-α. Cytosolic-to-nuclear translocation was observed in HASMC exposed to either E-Hb or TNF-α. Scale bar=25 μm.
