Figure 8.

(a) Representative experiment of the time-course detection of intracellular superoxide anions using the fluorescent probe DHE. Cultures were treated with either N-Hb or E-Hb (both at 10 nM) and 1 h later the fluorescent probe was added to HASMC. In some cases, E-Hb was added together with SOD (200 U ml⁻¹). (b) Effect of the ROS scavengers SOD (200 U ml⁻¹) and DMTU (1 mM) on the increase in planar cell surface area induced by 10 nM E-Hb. Results are expressed as means±s.e.m. of four independent experiments. ^*P<0.05 vs basal, ^†P<0.05 vs E-Hb.