Figure 4.

Imaging EGFP-positive blood vessels in living adult transgenic zebrafish. (a) Full-length view of a TG(fli1:EGFP)^y1 adult fish reveals robust EGFP expression throughout the animal. Higher magnification images from the same fish (b – d) show that the EGFP expression is largely restricted to blood vessels. (b) Merged transmitted light and fluorescent image from the tail fin, showing vessels running along and between fin rays. (c) Confocal image of tail fin microvasculature, showing vascular-specific EGFP expression. (d) Confocal image of blood vessels associated with surface scales in the same adult fish. The TG(fli1:EGFP)^y1 lines have proven useful in examining angiogenesis during the regeneration of severed tail fins in wild-type (e) or temperature-sensitive fin regeneration mutant (f) animals. Regenerating vessels in the mutant shown in panel (f) fail to branch properly and form enlarged vascular sinuses rather than properly patterned vascular plexuses as in wild-type animals. Panels a – d are from Lawson & Weinstein (2002b). Panels e and f are the courtesy of C.-C. Huang & S. Johnson.