Table 1.

Cpd 6A inhibits T cell cycle progression into S phase of the cell cycle

	% Cell
	G0/G1	S	G2/M
Condition
Unstimulated	95.3±5.5	1.6±4.2	3.1±2.5
Time after PHA stimulation (h)
0	92.7±4.7	1.4±2.6	5.9±3.0
2	91.8±3.8	4.3±4.0	3.9±2.7
4	93.0±3.2	3.9±2.9	3.1±1.0
6	91.8±2.7	2.8±1.2	5.4±4.9
8	91.3±1.6	5.9±0.4	2.8±3.8
12	86.4±2.1	8.9±2.4	4.7±1.6
16	85.1±3.0	11.2±2.5	3.7±2.9
24	77.8±2.6	16.8±3.1	5.4±1.8
36	66.8±3.9	24.7±2.6	8.5±0.8
48	63.3±5.5	27.9±3.9	8.8±4.3
72	61.9±3.8	29.5±4.2	8.6±5.3
Time of addition of 25 μM Cpd 6A after PHA stimulation (h)
0	95.5±4.8	0.9±0.5	3.6±3.0
2	95.6±3.2	2.2±2.0	2.2±1.8
4	97.9±3.9	1.4±0.8	0.7±0.3
6	93.4±4.5	1.9±1.8	4.7±3.9
8	94.7±5.1	2.6±1.9	2.7±1.7
12	89.7±2.6	3.7±2.1	6.6±5.0
16	91.3±7.0	3.3±3.0	5.4±1.2
24	88.9±6.9	5.0±2.9	6.1±3.3
36	80.0±4.2	14.3±3.5	5.7±4.4
48	74.3±1.5	23.3±2.2	6.4±1.3
72	61.9±1.0	30.0±3.2	8.1±5.6

In PHA controls, T cells (2 × 10⁶) were stimulated with PHA (5 μg ml⁻¹) and collected for cell cycle analysis at the indicated time. In experiment groups, after PHA stimulation, 25 μM of Cpd 6A was added at the indicated time and progression of cell cycle was analyzed at the third day. Analysis of cell cycle was performed as described in Methods. These data are representative of three separate experiments.