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. 2000 Feb 11;97(5):1988–1992. doi: 10.1073/pnas.040573197

Figure 6.

Figure 6

Interaction with targets in the transcriptional machinery. (A) The effect of overexpression of one activator on the activity of another. The overexpressed activator is called the squelcher, and the reporter is as indicated. (B) Interaction of Gal4-peptides with TBP, SRB4, and SRB10. Labeled Gal4(1–100), Gal4(1–100)+P201, or Gal4(1–100)+(840–881) were incubated with flag-tagged targets of Gal4 and then tested for their ability to coimmunoprecipitate with the targets (15). Input lane contains 10% of the TnT material used in each coimmunoprecipitation.