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. 2003 Sep 29;140(5):980–986. doi: 10.1038/sj.bjp.0705514

Figure 5.

Figure 5

Thrombin inhibition of IL-12 production is related to IL-10. PBMC (106 cells ml−1) were cultured in the presence of 150 nM thrombin and 40 μM SFLLRN peptide for 1 h at 37°C, then supplemented with FCS (1%) and anti-CD3 (0.1 μg ml−1)/anti-CD28 (1 μg ml−1), in the presence or absence of 10 μg ml−1 of anti-IL-10 (BT-10). After 16 h of culture, cell-free supernatants were obtained and IL-12 p70 present in the supernatants was determined by ELISA. The mean±s.e.m. of three independent experiments are presented. Asterisks indicate statistically significant (P<0.05) differences between IL-12 release in cultures treated with thrombin or thrombin derivatives versus controls, as determined using Student's t-test and Bonferroni's correction.