Figure 1.

(a) Typical experiment showing the effect of ceramide (10 μM) on myocyte shortening in isolated ventricular myocytes. Myocyte shortening and relengthening were recorded at 25°C before and 5 min after ceramide application. (b) Concentration-dependent response to ceramide (0–100 μM) on myocyte shortening in ventricular myocytes. Mean±s.e.m., n=33–41 cells per group, ^*P<0.05 vs control, as determined by repeated measures ANOVA and subsequent Tukey's post hoc test.