Table 1.

Effect of ceramide on baseline mechanical characteristics in cells from adult Sprague–Dawley rat hearts

	TPS (ms)	TR90 (ms)	+dLdt (μm s−1)	−dLdt (μm s−1)
Control	174±6	356±18	72.9±5.2	−66.1±6.7
Ceramide 10 pM	167±8	367±20	70.6±7.8	−58.0±9.5
Ceramide 1 nM	189±10*	296±21	89.8±6.8	−79.5±7.2
Ceramide 10 nM	212±8*	318±18	91.2±7.0*	−79.7±7.1
Ceramide 1 μM	218±8*	296±18*	97.4±7.7*	−102.4±11.0*
Ceramide 10 μM	213±9*	295±20*	93.1±6.9*	−91.4±8.1*
Ceramide 25 μM	211±9*	289±18*	96.3±6.5*	−97.5±8.3*
Ceramide 100 μM	209±9*	271±17*	101.2±7.6*	−98.6±7.8*

Time-to-PS (TPS), time-to-90% relengthening (TR₉₀), maximal velocities of shortening and relengthening (±dLdt). Data represent mean±s.e.m. n=32–41 cells per group.

^*P<0.05 vs control, as determined by repeated measures ANOVA and subsequent Tukey's post hoc test.