Table 1.
Summary of the effects of different agents or treatments on pEC50 values and maximal relaxations to H2O2 in PGF2α contracted mouse SMA
| Treatment | n | pEC50 | Maximal relaxationa (%) |
|---|---|---|---|
| Control (EC+) | 8 | 4.54±0.13 | 94.4±1 |
| Control (EC−) | 5 | 4.6±0.2 | 95.1±1 |
| Indomethacin 10 μM | 4 | 4.57±0.14 | 93.7±1 |
| ODQ 10 μM | 6 | 4.51±0.18 | 94.8±1 |
| EDTA 0.067 mM | 4 | 4.37±0.04 | 96.6±1 |
| 3-AT 50 mM | 4 | 4.58±0.03 | 93.2±1 |
| KCl 25 mM | 4 | 4.31±0.06 | 87.1±1b |
| TEA 10 mM | 4 | 4.27±0.06 | 94.8±1 |
| Ap 1 μM/ChTx 100 nM | 4 | 4.57±0.04 | 97.6±1 |
| 4-AP 1 mM | 4 | 4.38±0.11 | 93.8±1 |
| TEA/4-AP | 4 | 4.79±0.05 | 91.2±2 |
| Oua 100 μM/Ba2+ 30 μM | 4 | 4.52±0.1 | 90.3±5 |
a
Maximal relaxations are expressed as percentages of the contraction to PGF2α (0.01–0.1 μM).
b
P<0.05 when compared to control (EC+), ANOVA, followed by Dunnett's multiple comparison test.
3-AT: 3-amino-1,2,4-triazole; 4-AP: 4-aminopyridine; Ap: apamin; Ba2+: barium chloride; ChTx: charybdotoxin; EC+: endothelium-intact; EC−: endothelium-denuded; EDTA: ethylene diamine tetraacetic acid; H2O2: hydrogen peroxide; KCl: potassium chloride; ODQ: 1-H[1,2,4]oxadiazolo[4,3-]quinoxalin-1-one; oua: ouabain; pEC50: −log[EC50]; TEA: tetraethylammonium.