Figure 6.

Y-27632 and HA-1077 inhibited PDGF-induced chemotaxis. Cell migration was assessed using modified Boyden chambers with 8-μm pore filters. Serum-starved PSCs were left untreated (‘Cont') or were treated with PDGF-BB (at 25 ng ml⁻¹) in the lower chamber in the absence or presence of Y-27632 or HA-1077 (at 10 or 25 μM). After 24-h incubation with PDGF-BB, the cells migrated to the underside of the filter were stained, and viewed at × 200 magnification. Cell counts were obtained in six randomly chosen fields. Data are shown as mean+s.d. (n=6). **P<0.01 vs PDGF-BB only.