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Effect of SP600125, SB203580 and PD98059 on JNK, MAPKAPK-2 and ERK activities. Cells were stimulated with VT2 (3 ng ml−1) in the presence or absence of SP600125 (10 μM), SB203580 (10 μM) and PD98059 (50 μM) and were assayed for JNK activity (a, n=4) by solid-phase phosphorylation of GST-c-Jun, for MAPKAPK-2 activity (c) or whole-cell extracts were blotted for phospho-ERK (d). In (b, d and e) each value represents the mean±s.d. of at least three experiments.
