Table 2.
Comparative analysis of the kinetics of ER Ca2+ depletion and refilling in response to anandamide and bradykinin
ER Ca2+ kinetics (s) | ER Ca2+ depletion | ER Ca2+ refilling | τ of ER Ca2+ refilling (s) |
---|---|---|---|
Anandamide | 48.2±8.2 s* | 101.4±6.6 s* | 36±9.6 s* |
Bradykinin | 29.4±1.6 s | 241.6±10.4 s | 101.0±18.4 s |
ER Ca2+ depletion, the time until the ER was maximally depleted after the addition of the agonist; ER Ca2+ refilling, the time needed for ER refilling after agonist washout in Ca2+-containing buffer; τ of ER Ca2+ refilling, the time until the ER was 50% refilled after agonist washout in Ca2+-containing buffer. Each time represents the means±s.e.m.
P<0.05 vs bradykinin.