Table 3.
Autoradiographic distribution of [3H]mesulergine-binding sites corresponding to 5-HT7 receptors in human, rat and guinea-pig brain
| Specific binding* in fmol mg tissue−1 (n) | |||
|---|---|---|---|
| Human | Rat | Guinea-pig | |
| Olfactory bulb | |||
| Glomerular and external plexiform layers | 10.8±2.1 (6) | ||
| Cortex and claustrum | |||
| Frontal, outer layers (I–IV) | 2.1±0.4 (9) | 10.6±2.3 (4) | |
| Frontal, inner layers | 5.4±0.9 (11) | 7.7±2.8 (4) | |
| Frontoparietal, layers I–II | 8.5±1.1 (5) | ||
| Frontoparietal, layer III | 6.4±1.1 (5) | ||
| Frontoparietal, layer IV | 5.9±0.6 (5) | ||
| Frontoparietal, layer V | 5.7±1.2 (5) | ||
| Frontoparietal, layer VI | 2.9±1.7 (5) | ||
| Temporal, layers I–III | 7.2±2.2 (5) | 4.0±0.9 (5)a | |
| Temporal, layer IV | 7.5±1.3 (5) | ||
| Temporal, layer V | 6.8±1.3 (5) | 4.3±0.9 (5) | |
| Temporal, layer VI | 6.6±1.7 (5) | ||
| Occipital | 7.6±1.4 (6) | ||
| Entorhinal, layers I–III | 4.3±2.0 (5) | 11.1±2.3 (4) | 8.5±3.0 (4) |
| Entorhinal, layers IV–VI | 7.5±2.8 (5) | ||
| Primary olfactory | 17.2±2.5 (5) | ||
| Claustrum | 1.6±0.5 (5) | 10.3±1.1 (4) | 15.2±2.0 (7) |
| Basal ganglia and olfactory tubercle | |||
| Nucleus accumbens | 6.8±2.5 (6) | 8.6±1.6 (6) | |
| Internal capsule | 1.7±0.7 (6) | ||
| Caudate nucleus | 9.7±1.9 (11) | 7.1±1.8 (5)b | 9.0±2.9 (4)b |
| Putamen nucleus | 9.4±2.0 (9) | ||
| Globus pallidus | 7.2±1.7 (5) | 8.8±2.1 (6) | |
| Olfactory tubercle | 9.3±1.4 (4) | 42.9±10.7 (7) | |
| Hippocampus and septum | |||
| Field 1 of Ammon's horn, oriens layer | 4.0±1.6 (6) | 11.1±2.5 (5)c | 7.0±2.2 (7)c |
| Field 1 of Ammon's horn, pyramidal layer | 5.6±2.6 (6) | ||
| Field 1 of Ammon's horn, radial layer | 4.9±1.9 (7) | ||
| Field 2 of Ammon's horn, oriens layer | 3.6±2.0 (6) | 11.8±2.0 (5)c | 8.6±1.7 (5)c |
| Field 2 of Ammon's horn, pyramidal layer | 7.3±2.2 (7) | ||
| Field 2 of Ammon's horn, radial lacunosum-moleculare layer | 5.1±2.2 (8) | ||
| Field 3 of Ammon's horn | 13.1±1.8 (5) | ||
| Dentate gyrus, molecular layer | 6.6±2.1 (8) | 8.9±1.5 (5) | 13.8±2.4 (7) |
| Parasubiculum | 2.3±1.0 (7) | ||
| Presubiculum | 4.3±2.0 (6) | ||
| Subiculum dorsalis | 4.1±1.6 (8) | 8.2±0.8 (5) | |
| Subiculum ventralis | 25.6±2.6 (5) | 29.5±4.4 (4) | |
| Medial septal nucleus | 9.7±1.4 (7) | ||
| Lateral septal nucleus | 10.5±1.1 (4) | 6.6±2.0 (6) | |
| Thalamus | |||
| Anterior thalamic nucleus | 4.2±2.4 (6) | ||
| Reuniens nucleus | 25.3±2.7 (5) | 20.7±3.1 (6) | |
| Paratenial | 13.4±2.8 (6) | ||
| Paraventricular nucleus, posterior part | 30.3±5.2 (5) | 16.0±3.3 (7) | |
| Medial thalamic nucleus | 5.6±1.6 (5) | 14.0±3.0 (5)d | 17.4±2.1 (7)d |
| Centromedial nucleus | 11.6±0.6 (3) | 28.4±1.4 (5) | 17.5±2.7 (7) |
| Ventral anterior nucleus | 6.3±1.8 (7) | ||
| Ventroposterior nucleus, lateral part | 6.9±1.0 (5) | 12.6±3.1 (7) | |
| Ventromedial nucleus | 3.1±1.1 (4) | 9.8±0.6 (5) | 14.9±1.0 (3) |
| Lateroanterior thalamic nucleus | 2.2±1.1 (3) | ||
| Laterodorsal, ventrolateral | 13.2±2.3 (5) | 9.1±1.1 (6) | |
| Laterodorsal, dorsomedial part | 8.1±1.0 (6) | ||
| Posterior nuclear group | 11.7±1.8 (5) | 12.9±2.0 (6) | |
| Reticularis nucleus | 3.3±2.0 (5) | 11.7±3.2 (5) | |
| Subthalamic nucleus | 6.7±3.9 (4) | ||
| Anterior pretectal area | 8.3±1.6 (5) | 16.1±1.2 (4) | |
| Medial geniculate nucleus | 12.2±1.7 (5) | ||
| Ventrolateral geniculate nucleus | 15.0±3.2 (4) | ||
| Hypothalamus | |||
| Arcuate nucleus | 14.0±0.9 (5) | 15.4±2.5 (5) | |
| Anterior hypothalamic area | 13.2±1.4 (5) | ||
| Lateral area | 10.1±1.1 (5) | ||
| Medial preoptic area | 14.1±2.3 (5) | 9.3±2.1 (4) | |
| Ventromedial nucleus | 11.5±1.7 (5) | ||
| Dorsomedial nucleus | 14.9±1.6 (5) | ||
| Posterior hypothalamic area | 32.9±18.6 (5) | ||
| Mammillary nucleus | 56.9±16.0 (6) | ||
| Suprachiasmatic nucleus | 17.6±2.1 (4) | ||
| Midbrain, pons, and medulla | |||
| Superficial gray layer of the superior colliculus | 6.6±1.6 (4) | 17.8±1.6 (5) | 9.4±1.6 (6) |
| Optic nerve layer of the superior colliculus | 12.2±1.1 (5) | 7.1±1.5 (6) | |
| Intermediate gray layer of the superior colliculus | 12.8±2.2 (5) | 10.8±1.6 (6) | |
| Central (periaqueductal) gray | 7.9±1.7 (6) | 14.9±0.7 (5) | 17.0±2.8 (5) |
| Deep mesencephalic nucleus | 7.4±1.3 (4) | 11.4±1.2 (7) | |
| Caudate linearis nucleus | 6.3±2.0 (5) | ||
| Dorsal raphe nucleus | 9.7±3.3 (7) | 14.2±2.8 (4) | 16.9±3.3 (5) |
| Median raphe nucleus | 3.7±2.4 (3) | ||
| Medial lemniscus | 15.6±2.6 (5) | ||
| Red nucleus | 7.7±3.1 (6) | 9.2±2.0 (4) | |
| Interpeduncular nucleus | 15.0±3.2 (5) | 26.6±5.7 (7) | |
| Pontine nucleus | 3.8±0.4 (4) | ||
| Substantia nigra | 14.2±4.2 (7) | 5.4±1.5 (5) | 13.1±1.5 (6) |
*
Specific binding of 10 nm [3H]mesulergine in the presence of 1 μM raclopride and 0.8 μm DOI transformed in Bmax considering a KD value of 10 nm.
a
Outer layers.
b
Caudate-putamen.
c
All layers of the hippocampal field.
d
Mediodorsal nucleus.