Table 3.
Effect of blockers of neuronal voltage-gated Ca2+ channels, capsaicin-sensitive primary afferents, NO synthase and guanylate cyclase on the relaxations to PACAP 38
| n | pD2 | Emax (%) | |
|---|---|---|---|
| Control | 6 | 7.53±0.11 | 86.7±6.3 |
| ω-CgTX (1 μM) | 6 | — | 63.7±7.8* |
| Control | 8 | 7.95±0.08 | 67.7±6.9 |
| Capsaicin (10 μM) | 8 | — | 40.7±5.7* |
| Control | 6 | 7.96±0.12 | 65.2±7.0 |
| L-NOARG (100 μM) | 6 | — | 43.6±7.2* |
| Control | 6 | 7.68±0.20 | 85.5±4.7 |
| ODQ (5 μM) | 6 | — | 70.7±4.3* |
The results are expressed as mean ±s.e.m. of n experiments.
*
Parameter significantly (P<0.05) different compared (paired t-test) to the control value. Emax is the maximal relaxation, expressed as a percentage of the U46619-induced contraction, obtained for each drug. pD2=−log EC50, where EC50 is the concentration of agonist producing 50% of the Emax.