Figure 2.

Time-dependent effect of IL-1β (a, d) (400 pg ml⁻¹; hatched pale grey columns), budesonide (b, e) (0.1 μM; hatched black columns) and IL-1β + budesonide (c, f) (hatched dark grey columns) in the presence of RU486 (1 μM) on SCF mRNA (a–c) and protein (d–f) expression by human lung fibroblasts in culture. SCF¹⁴⁹ cDNA was quantified after total RNA RT by on-line fluorescent PCR. Results were normalised to total cDNA and expressed as fg SCF¹⁴⁹ cDNA ng total cDNA⁻¹. SCF protein levels were assessed in the supernatant by ELISA and the are expressed in pg ml⁻¹. Results are mean values (blocks)±s.e.m. (bars) of four experiments performed in duplicate on fibroblasts from three different donors. Values after treatment were expressed as a percent of variation (%) of SCF¹⁴⁹ cDNA or protein levels from fibroblasts treated with solvent alone for the same time period.