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Effect of IL-1β+budesonide on SCF mRNA transcription rate. Fibroblasts were treated with ethanol (1 μM: control) or IL-1β (400 pg ml−1)+budesonide (0.1 μM) for 30 min. Nuclei were prepared, and SCF gene transcription rate was assessed after autoradiography of blots (top), and by the ratio of SCF mRNA signal to GAPDH mRNA signal (bottom), representative of two experiments perfomed on fibroblasts from two different donors.
