Figure 2.

Western blot analysis of PLB expression levels in microsomal fractions enriched in SR membranes isolated from control, diabetic, and insulin-treated diabetic rat hearts. (a) Representative Western blots using the anti-mouse PLB monoclonal antibody show that PLB monomers and pentamers migrated at ∼5 and ∼25 kDa on SDS–PAGE, respectively (right panel). When the microsomal preparations were boiled for 5 min immediately before electrophoresis, all the PLB migrated as the monomeric form (left panel). (b) Quantification of total PLB protein levels, detected as the monomeric form after boiling. In each of the experiments, the SERCA2a level obtained from the control band is normalized as 1.0. Bars are means±s.e.m. of four separate experiments. ^*P<0.05, ^***P<0.001.