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Inducible overexpression of GRK5. EcR293:β2-adrenoceptor:GRK5 cells were grown for 72 h in the presence of 0.125% ethanol (EtOH) or varying concentrations of ponasterone A (Pon A) up to 10 μM. The control lane (Con) is a lysate of EcR293:β2-adrenoceptor cells not transfected with GRK5. GRK5 migrates at approximately 69 kDa.
