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. 2003 Dec 22;141(2):277–284. doi: 10.1038/sj.bjp.0705504

Figure 6.

Figure 6

β2-adrenoceptor internalization and recycling in HEK293 cells transiently coexpressing receptors and GRK5. HEK293 cells were transfected with β2-adrenoceptor cDNA and GRK5 or plasmid cDNA. (a) Total cell extracts from GRK5-transfected and control cells were probed with anti-GRK5 antibody. (b) Receptor internalization and recycling were determined following treatment with isoprenaline (10 μM) for 30 min at 37°C. Each point represents the means±s.e.m. of three independent experiments.