Table 1.
Endocytic and recycling rate constants (min−1) in EcR293:β2-adrenoceptor cell lines
ke | kr (15 min) | kr (6 h) | |
---|---|---|---|
Untransfected | 0.168±0.013 | 0.121±0.009 | 0.086±0.007 |
GRK5 (EtOH) | 0.229±0.013 | 0.139±0.009 | 0.118±0.013 |
GRK5 (PON) | 0.197±0.009 | 0.124±0.011 | 0.085±0.010 |
Individual rate constants for β2-adrenoceptor endocytosis (ke) and recycling (kr) following 15 min and 6 h exposures to isoprenaline were determined as previously described (Morrison et al., 1996). Rate constants are expressed as means±s.e.m. from three independent experiments.