Figure 5.

Effects of NECA and CPA on pH_i in HCPSC. Cells were incubated with the BCECF-AM (10 μM) prior to the addition of either agonist. Panel (a) shows the concentration response curves to NECA and CPA, measuring changes in pH_i (30 min after agonist addition, n=6). Panel (b) shows the effect of adenosine deaminase (0.1 U ml⁻¹) on the control pH_i and the response to NECA (1 μM) (n=4 for both). ^*Significantly different when compared to vehicle control (VC) (P<0.05; one-way ANOVA). +Significantly different when compared to adenosine deaminase (AD 0.1 .U ml⁻¹) (P<0.05, Bonferroni's test). NS, not significant.