Figure 2.

Role of ROS in CEES-induced death of Jurkat cells. (a) Jurkat cells were incubated first for 1 h with or without 25 μM H₂O₂, and then for 6 h in the additional absence or presence of 600 μM CEES, after which cell viability was assessed by measurement of calcein-AM fluorescence. (b) Cells were incubated with CEES (600 μM) for the indicated times, after which ROS generation was measured by fluorometric analysis of H₂DCF oxidation. (c) Cells were incubated first for 1 h with or without 1 mM GSH-ethyl ester (GSHE) or 5 mM NAC, and then for 5 h in the additional absence or presence of 600 μM CEES, after which ROS generation was determined. All data are means±s.d. of values from an experiment that was repeated three times with similar results. ^*P<0.05 versus control value for untreated cells.