Figure 4.

Effects of thiol antioxidants and a PARP inhibitor on CEES-induced cytotoxicity. (a) Jurkat cells (closed bars) and normal lymphocytes (open bars) were incubated first for 1 h with or without 5 mM NAC, 1 mM GSH-ethyl ester, or 5 mM 3-AB, and then for 18 h in the additional absence or presence of 600 μM CEES, after which cell viability was assessed by measurement of calcein-AM fluorescence. (b) Jurkat cells were incubated first for 1 h with or without NAC or GSH-ethyl ester, and then for 6 h in the additional absence or presence of CEES, after which the intracellular concentration of GSH was determined. All data are means±s.d. of triplicates from an experiment that was repeated a total of three times with similar results. ^*P<0.05 versus the control value for untreated cells.